Cell-surface radioiodination with the sparingly.soluble catalyst lodogen
ثبت نشده
چکیده
The surface proteins of dividing and non-dividing subpopulations of rat and mouse thymocytes have been labelled by using a new method of radioiodination. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and autoradiography of the labelled proteins shows distinct differences in labelling between the mouse and rat cells and also, in the case of the rat, between the dividing and non-dividing populations.
منابع مشابه
Pradeep K . Garg , Kevin L . Alston , et al . - Succinimidyl 5 - Iodo - 3 - Pyridinecarboxylate N Radioiodination of Internalizing Monoclonal Antibodies Using Updated
Monoclonal antibodies (mAbs) that internalize following binding to cell-surface receptors require radiolabeling approaches that minimize loss of radioactivity from the cell after intracellular processing. One class of internalizing mAbs ofgreat interest for imaging and radioimmunotherapy are those specific for EGERvIII, a truncated form ofthe epidermal growth factor receptor found on gliomas, n...
متن کاملIdentification and characterization of Vibrio cholerae surface proteins by radioiodination.
Whole cells and isolated outer membrane from Vibrio cholerae (Classical, Inaba) were radiolabeled with Iodogen or Iodo-beads as catalyst. Radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. Surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine w...
متن کاملThree different radioiodination methods for human spleen ferritin compared.
We compared three different methods for radioiodination of human spleen ferritin. Tracers prepared by direct oxidative iodination with use of Chloramine T or 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) were structurally altered by the labeling procedure, as was made apparent by gel filtration and electrophoresis on polyacrylamide gel. Tracers prepared by conjugation to rad...
متن کاملPlatinum Nanoparticles Deposited on Oxygen-Containing Functional Groups at Carbon Vulcane XC-72 as a Cathode Catalyst for Direct Methanol Fuel Cell
Surface oxidized carbon vulcane XC-72 is prepared as catalyst support and platinumnanoparticles are chemically anchored onto the modified surface. The nanoparticles of Pt weresynthesized by reduction of H2PtCl6 with sodium borohydride in a 5.5 M buffer solution ofsodium citrate; the complexation of citrate with metal ions is beneficial to the formation ofnanoparticles. The electro-oxidation of ...
متن کاملThe Impact of Wettability on Effective Properties of Cathode Catalyst Layer in a Proton Exchange Membrane Fuel Cell
The produced liquid water in cathode catalyst layer (CCL) has significant effect on the operation of proton exchange membrane fuel cell (PEMFC). To investigate this effect, the transport of oxygen in CCL in the presence of immiscible liquid water is studied applying a two-dimensional pore scale model. The CCL was reconstructed as an agglomerated system. To explore the wettability effects, diffe...
متن کامل